| 摘要: |
| [目的] 探讨黄元灸对脾虚型慢性疲劳综合征（CFS）大鼠线粒体能量代谢的影响，明确其作用机制。[方法] 将40只SPF级雄性SD大鼠适应性喂养1周后随机分为正常组、模型组、药饼组（黄元灸组）、悬灸组、淀粉组（各8只）。采用慢性束缚、力竭游泳及高乳糖饲料喂养，建立脾虚型CFS模型。取关元穴施灸，药饼组隔药饼（党参∶茯苓∶干姜∶炙甘草=3∶2∶2∶2）灸；悬灸组行温和悬灸；淀粉组隔淀粉饼灸。干预5 d后，检测以下指标：一般情况评分、体重变化量；力竭游泳时间、悬尾实验数据、水迷宫实验数据；肝、小肠、骨骼肌、海马组织的ATP含量及钠钾、钙镁ATP酶活性；透射电镜观察线粒体形态。[结果] 药饼组在一般情况评分显著优于模型组和悬灸组（P<0.01），体质量上升更明显（P<0.01）；力竭游泳平均时间最长（5.45 min），悬尾不动平均时间最短（25.62 s），运动耐力接近正常组；水迷宫中药饼组运动速度显著快于淀粉组和悬灸组（P<0.05）。药饼组在肝组织中的钙镁ATP酶活性显著高于模型组（P=0.049），在小肠、骨骼肌、海马组织中低于模型组；电镜下药饼组线粒体结构损伤显著改善，并出现自噬小体（双层膜包裹嵴碎片）。[结论] 黄元灸在改善脾虚型CFS大鼠整体状态及部分能量代谢指标上展现出优于悬灸和淀粉灸的趋势，黄元灸通过双向调节钙镁ATP酶活性及激活线粒体自噬，修复线粒体结构损伤，改善能量代谢紊乱，从而治疗脾虚型CFS。 |
| 关键词: 黄元灸 慢性疲劳综合征 ATP 钙镁ATP酶 |
| DOI：10.11656/j.issn.1673-9043.2026.08.08 |
| 分类号:R245.8;R255.5 |
| 基金项目:河南省“双一流”创建学科中医学科学研究专项（HSRP-DFCTCM-2023-2-20）。 |
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| Effect of Huangyuan moxibustion on mitochondrial energy metabolism in rats with chronic fatigue syndrome of spleen deficiency type |
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HU Yuming, LYU Peiwan, YANG Shuai, YANG Zhaoyun, LI Yingying, CHENG Yajie
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The Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China
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| Abstract: |
| [Objective] To investigate the effects of Huangyuan moxibustion on mitochondrial energy metabolism in rats with spleen deficiency-type chronic fatigue syndrome（CFS）and to clarify its mechanism of action. [Methods] Forty SPFgrade male SD rats were randomly divided into five groups after one week of adaptive feeding：normal group，model group，herbal cake group（Huangyuan moxibustion group），suspended moxibustion group，and starch cake group，with 8 rats in each group. A spleen deficiency-type CFS model was established by chronic restraint， exhaustive swimming，and high-lactose diet feeding. Moxibustion was applied at Guanyuan（CV4）acupoint. The herbal cake group received moxibustion with a herbal cake composed of Codonopsis Radix，Poria，Zingiberis Rhizoma，and Glycyrrhizae Radix et Rhizoma Praeparata（3∶2∶2∶2）. The suspended moxibustion group received mild suspended moxibustion. The starch cake group received moxibustion with a starch cake. After 5 days of intervention，the following indicators were measured：general condition score，body weight change，exhaustive swimming time，tail suspension test data，Morris water maze data，ATP content and Na+/K+-ATPase and Ca2+/Mg2+-ATPase activities in liver，small intestine，skeletal muscle，and hippocampus tissues，and mitochondrial morphology observed by transmission electron microscopy. [Results] The herbal cake group showed significantly better general condition scores than the model group and suspended moxibustion group（P<0.01），with more pronounced weight gain（P<0.01）. The average exhaustive swimming time was longest（5.45 min）and the average immobility time in the tail suspension test was shortest（25.62 s）in the herbal cake group，with exercise endurance approaching that of the normal group. In the Morris water maze，the herbal cake group showed significantly faster swimming speed than the starch cake group and suspended moxibustion group（P<0.05）. The Ca2+/Mg2+-ATPase activity in liver tissue of the herbal cake group was significantly higher than that of the model group（P=0.049），while it was lower than the model group in small intestine，skeletal muscle，and hippocampus tissues. Under electron microscopy，mitochondrial structural damage in the herbal cake group was significantly improved，and autophagosomes（double membrane structures containing cristae fragments）were observed. [Conclusion] Huangyuan moxibustion showed a tendency to be superior to suspended moxibustion and starch cake moxibustion in improving the overall status and some energy metabolism indicators in rats with spleen deficiencytype CFS. Huangyuan moxibustion may treat spleen deficiency type CFS by bidirectionally regulating Ca2+/Mg2+ ATPase activity and activating mitophagy to repair mitochondrial structural damage and improve energy metabolism disorders. |
| Key words: Huangyuan moxibustion chronic fatigue syndrome ATP calcium-magnesium ATPase |
