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| Optimization of the extraction and purification process for total flavonoids from Artemisia capillaris Thunb. （flowering stage）and investigation of their antioxidant efficacy |
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| DOI
10.11656/j.issn.1673-9043.2026.07.05 |
| Key Words
Artemisia capillaris Thunb.;total flavonoids;macroporous resin;xanthine oxidase;antioxidant activity |
| Author Name | Affiliation | E-mail | | LIU Mingzhu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Xueyuan | Shengshi Baicao Pharmaceutical Co. Ltd., Tianjin 300301, China | | | TAN Weihao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SUN Lufu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Naijiao | Shengshi Baicao Pharmaceutical Co. Ltd., Tianjin 300301, China | | | LI Tianxiang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | litianxiang612@sina.com |
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| Abstract
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| [Objective] To optimize the extraction and purification process of total flavonoids from Artemisia capillaris Thunb（. flowering stage）（TFHC）and evaluate their in vitro antioxidant activity，providing an experimental basis for the industrial preparation and pharmacological application of TFHC. [Methods] The extraction process of TFHC was optimized by ultrasound-assisted hot maceration combined with orthogonal experiment and response surface methodology. Six macroporous resins（NKA-9，HPD-600，AB-8，DM-130，D-101，D-4020）were screened for dynamic purification to obtain refined TFHC. High-performance liquid chromatography（HPLC） and UV spectrophotometry were used to systematically evaluate the DPPH radical scavenging capacity and xanthine oxidase （XOD）inhibitory activity of the purified product. [Results] The optimal extraction parameters were determined as follows：liquid-to-solid ratio of 51 mL/g，ethanol volume fraction of 38.2%，ultrasonication time of 30 min，hot maceration temperature of 70 ℃，and hot maceration time of 1 h. D-101 resin exhibited the best static adsorption and desorption capacity. The optimal purification process was：loading concentration of 1.5 mg/mL，loading flow rate of 7 BV/h，resin column diameter-to-height ratio of 1∶12，loading volume of 2.35 BV，impurity elution volume of 2.94 BV，eluent of 70% ethanol，elution flow rate of 10.6 BV/h，and eluent volume of 2.35 BV. After the optimal purification process，the purity of TFHC increased from 38.33% to 92.00%. The half-maximal inhibitory concentration（IC50）of purified TFHC against XOD decreased from 5.061 mg/mL to 1.905 mg/mL，and the IC50 for DPPH radical scavenging capacity decreased from 0.070 mg/mL to 0.033 mg/mL. [Conclusion] The optimized extraction and purification process significantly improved the yield and purity of TFHC. The purified product showed enhanced DPPH radical scavenging capacity and XOD inhibitory activity，demonstrating potent antioxidant activity. |
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