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| Protective effect of kaempferol on rats with acute ischemic stroke by regulating Wnt/β-catenin signaling pathway |
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| DOI
10.11656/j.issn.1673-9043.2026.07.07 |
| Key Words
kaempferol;acute ischemic stroke;neurological function;inflammation;wingless MMTV integration site family;β-catenin |
| Author Name | Affiliation | E-mail | | LI Xiangnan | Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | | | YANG Songtao | Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | | | LI Lin | Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | | | ZHANG Zhiyong | Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | | | LI Jianmin | Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | | | ZHANG Yunhe | Department of Neurosurgery, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | zhangyunhe1973@sohu.com | | LI Rui | Department of Neurology, Affiliated Hospital of North China University of Science and Technology, Tangshan 063015, China | |
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| Abstract
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| [Objective] To explore the effects of kaempferol on neurological function，inflammation，cerebral infarction volume and pathological damage of brain tissue in rats with acute ischemic stroke（AIS），and analyze whether the regulatory effect on the wingless MMTV integration site family（Wnt）/β-catenin signaling pathway is its mechanism of action. [Methods] The rat model of AIS was established by middle cerebral artery embolization and reperfusion. The 72 successfully modeled AIS rats were randomly divided into model group，kaempferol group （50 mg/kg），Wnt/β-catenin pathway inhibitor group（ICG-001，5 mg/kg），kaempferol+Wnt/β-catenin pathway inhibitor group（50 mg/kg + 5 mg/kg），with 18 rats in each group. Another 18 rats were selected as the sham operation group（performing the same surgical operations without inserting the suture plugs）. Each group received corresponding intervention for 14 days. The neurological function impairment of rats was evaluated by the Zea-Longa score；the levels of serum inflammatory factors [tumor necrosis factor-α（TNF-α），monocyte chemoattractant protein-1（MCP-1），NOD-like receptor thermal protein domain-associated protein 3（NLRP3），interleukin-1β （IL-1β）] were determined by enzyme-linked immunosorbent assay；2，3，5-triphenyltetrazolium chloride staining was used to determine the percentage of cerebral infarction volume in rats；hematoxylin-eosin staining was used to observe the pathological changes of brain tissue in the ischemic cortical area of rats；the expressions of Wnt1， Wnt3a，β-catenin messenger RNA（mRNA）and protein in the brain tissue of the ischemic cortical area of rats were determined by fluorescence quantitative PCR and Western blotting，respectively. [Results] After 14 days administration，compared with sham operation group，the neuronal nuclei in the brain tissue of the ischemic cortical area of rats in model group were deformed and constricted，the arrangement was looser，the cells were polygonal，and necrotic cells could be seen，the neurological function impairment score，the levels of serum TNF-α，MCP-1， NLRP3，IL-1β，and the percentage of cerebral infarction volume were significantly increased，the Wnt1，Wnt3a，β-catenin mRNA and protein expression levels in the brain tissue of the ischemic cortical area were significantly decreased（P<0.05）. Compared with model group，the phenomena of neuronal nucleus deformation and condensation，loose arrangement and cell necrosis in the brain tissue of the ischemic cortical area of rats in kaempferol group were significantly improved，the neurological function impairment score，the levels of serum TNF-α，MCP-1，NLRP3，IL-1β，and the percentage of cerebral infarction volume were significantly decreased，the Wnt1， Wnt3a，β-catenin mRNA and protein expression levels in the brain tissue of the ischemic cortical area were significantly increased（P<0.05）；in the Wnt/β-catenin pathway inhibitor group，the phenomena of neuronal nucleus deformation and condensation，loose arrangement and cell necrosis in the brain tissue of the ischemic cortical area of rats were aggravated，the neurological function impairment score，the levels of serum TNF-α，MCP-1，NLRP3，IL-1β，and the percentage of cerebral infarction volume were significantly increased，the Wnt1，Wnt3a，β-catenin mRNA and protein expression levels in the brain tissue of the ischemic cortical area were significantly decreased（P <0.05）. The pathological damage of brain tissue in the ischemic cortical area and the above quantitative indicators of rats in the kaempferol+Wnt/β-catenin pathway inhibitor group were worse than those in the kaempferol group and better than those in the Wnt/β-catenin pathway inhibitor group（P<0.05）. [Conclusion] The activation effect on the Wnt/β-catenin signaling pathway may be the mechanism by which kaempferol improves neurological function， inflammation，cerebral infarction volume and pathological damage of brain tissue in AIS rats. |
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