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| Spectrum-effect relationship between HPLC fingerprint and in vitro antioxidant activity of different polar extracts from Rhaponticum repens |
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| DOI
10.11656/j.issn.1673-9043.2026.07.08 |
| Key Words
Rhaponticum repens;different polar extract;fingerprint;antioxidant activity;spectrum-effect relationship |
| Author Name | Affiliation | E-mail | | DING Man | Key Laboratory of Xinjiang Uygur Medicine, Xinjiang Institute of Materia Medica, Urumqi 830010, China | | | MAO Yan | Key Laboratory of Xinjiang Uygur Medicine, Xinjiang Institute of Materia Medica, Urumqi 830010, China | maoyan7529@163.com | | ADINA Abuduaini | Key Laboratory of Xinjiang Uygur Medicine, Xinjiang Institute of Materia Medica, Urumqi 830010, China
School of Pharmaceutical Sciences, Xinjiang Medical University, Urumqi 830011, China | | | WANG Xintang | Key Laboratory of Xinjiang Uygur Medicine, Xinjiang Institute of Materia Medica, Urumqi 830010, China | Email:wangxintang620@126.com |
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| Abstract
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| [Objective] To establish the HPLC fingerprints of different polar extracts from Rhaponticum repens and investigate their spectrum-effect relationship with antioxidant activity，preliminarily identifying the effective components contributing to their antioxidant activity. [Methods] Ultra-performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）was used to qualitatively analyze the chemical components of Rhaponticum repens. HPLC was used to establish fingerprints of different polar extracts of Rhaponticum repens. The antioxidant activities of different extracts were evaluated by the DPPH radical scavenging rate and total reducing capacity assays. The relationships between common peaks and antioxidant activity were established by partial least squares analysis and gray relational analysis，the effective components of antioxidant activity were screened，and in vitro antioxidant validation tests were carried out. [Results] Twenty-two common peaks were identified from the fingerprint profiles of eight different polar extracts of Rhaponticum repens，and seven chemical components were identified based on mass spectrometry results. The antioxidant activity results showed that different polar extracts exhibited different antioxidant activities. Among them，the ethyl acetate fraction and n-butanol fraction of the 50% ethanol extract had the strongest antioxidant activity. Analysis of the spectrum-effect relationship showed that the chemical components represented by peaks 1（neochlorogenic acid），2（chlorogenic acid），3（cryptochlorogenic acid），5，7，and 13 contributed the most to the antioxidant activity of Rhaponticum repens. The validation results of active ingredients showed that chlorogenic acid，neochlorogenic acid，and cryptochlorogenic acid possessed antioxidant activity in a concentration-dependent manner. [Conclusion] The antioxidant activity of Rhaponticum repens results from the synergistic effects of multiple components，with chlorogenic acid，neochlorogenic acid，and cryptochlorogenic acid being the primary active compounds. These findings provide a reference for elucidating the material basis of its antioxidant efficacy，as well as for quality control and further development and utilization of the medicinal material. |
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