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| Effect of Hejie Shengfa Decoction on immune cell subsets and hair regeneration in mice with alopecia areata |
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| DOI
10.11656/j.issn.1673-9043.2026.07.10 |
| Key Words
alopecia areata;Hejie Shengfa Decoction;immune cell subset;CD8+ cell |
| Author Name | Affiliation | E-mail | | ZHAO Lingling | Fourth Clinical Medical College, Xinjiang Medical University, Urumqi 830000, China | | | AIKEDA Tiemuer | Fourth Clinical Medical College, Xinjiang Medical University, Urumqi 830000, China | | | LIU Yushi | Fourth Clinical Medical College, Xinjiang Medical University, Urumqi 830000, China | | | ZHANG Chenghui | Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine, Urumqi 830000, China | 470703367@qq.com |
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| Abstract
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| [Objective] To investigate the effects of Hejie Shengfa Decoction（HJSFD）on hair regrowth and immune cell subsets in a mouse model of alopecia areata（AA），and to elucidate its potential immunoregulatory mechanisms. [Methods] An AA model was established in C3H/HeJ mice by topical application of imiquimod. The mice were randomly assigned to a blank control group，model group，low-，medium-，and high-dose HJSFD groups，and a compound glycyrrhizin tablet（CGT）group，with six mice in each group. All animals received intragastric administration for 14 consecutive days. Hair regrowth was evaluated by body weight monitoring，dermoscopy，H&E staining，and Ki67 immunofluorescence. Flow cytometry was used to detect peripheral CD8+ T cells，Th1，Th17，Treg cells，and the Th17/Treg ratio. [Results] Mice in the model group exhibited typical AA manifestations，including epidermal thickening，reduced hair follicles，and lymphocytic infiltration. Compared with the model group，HJSFD promoted hair regeneration in a mouse model of alopecia areata，ameliorated pathological alterations in hair follicle structure， and enhanced Ki67 expression in dermal papilla cells，decreased CD8+ T cells，Th1，and Th17 cells，elevated Treg cell levels，and restored the Th17/Treg balance. Among the treatment groups，the high-dose HJSFD group demonstrated the most pronounced therapeutic effects. [Conclusion] HJSFD promotes hair regrowth in AA mice， potentially by inhibiting the activation of CD8+，Th1，and Th17 cells，enhancing Treg-mediated immunoregulation， and restoring Th17/Treg homeostasis. These findings provide experimental evidence for the immunological mechanism of traditional Chinese medicine in the treatment of AA. |
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