| 摘要: |
| [目的]研究细胞培养基中人参皂苷Rg1和黄芩苷稳定性问题。[方法]采用高效液相色谱法测定细胞培养基中人参皂苷Rg1和黄芩苷的含量。[结果]人参皂苷Rg1和黄芩苷的线性范围均为20～400ng,方法回收率分别为96.8%和97.7%。[结论]该法简便、灵敏、特异,适用于细胞培养基中人参皂苷Rg1和黄芩苷的测定。 |
| 关键词: 培养基 人参皂苷Rg1 黄芩苷 |
| DOI：10.11656/j.issn.1672-1519.2006.02.31 |
| 分类号: |
| 基金项目:国家自然科学基金课题(30472177)。 |
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| Determination of Contents of Ginsenosides Rg1 and Baicalin in Cell Culture Medium with HPLC |
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LI Yue, DU Rong, ZHANG Yan-jun
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Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To study the stability of ginsenosides Rg1 and baicalin in culture medium.[Methods] Ginsenosides Rg1 and Baicalin in culture medium were determined with HPLC.[Results] The linear regressive curves of both substances were in the range of 20～400 ng. The recovery rates of Rg1 and Baicalin were 96.8% and 97.5% respectively.[Conclusions] This method is specific, simple, sensitive and suitable for the measurement of Ginsenosides Rg1 and Baicalin in culture medium. |
| Key words: culture medium ginsenoside Rg1 baicalin |
