| 摘要: |
| [目的]探讨丹参酮ⅡA对血管平滑肌细胞单核细胞趋化蛋白-1(MCP-1)、白细胞介素-1β(IL-1β)表达的影响。[方法]培养大鼠主动脉平滑肌细胞,复制肿瘤坏死因子-α(TNF-α)损伤模型,逆转录-聚合酶链反应(RT-PCR)、放射性免疫方法检测MCP-1、IL-1β的表达,并观察丹参酮ⅡA的作用。[结果]丹参酮ⅡA能够抑制TNF-α诱导的血管平滑肌细胞MCP-1、IL-1β的过度表达。[结论]抑制动脉粥样硬化过程中MCP-1、IL-1β的表达可能是丹参酮ⅡA的作用机制之一。 |
| 关键词: 丹参酮ⅡA MCP-1 IL-1β 实验研究 |
| DOI：10.11656/j.issn.1672-1519.2006.03.05 |
| 分类号: |
| 基金项目:天津市自然科学基金资助项目(023611611) |
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| Effect of Tanshinone ⅡA on Expression of MCP-1 and IL-1β in Injured Smooth Muscle Cell Induced by TNF-α |
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ZHOU Gui-tong,ZHAO Gui-feng,FAN Ying-chang
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Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To explore the influence of Tanshinone ⅡA on the expression of MCP-1 and IL-1β in the vascular smooth muscle cell. [Methods] Cultivate the aorta smooth muscle cell of rats and reproduce the TNF-α injured model. The expression of MCP-1 and IL-1β was determined by RT-PCR and radioimmunology assay and the effect of tanshinone ⅡA was observed. [Results] The transhinone ⅡA may inhibit MCP-1 and IL-1β overexpression of vascular smooth muscle cell injured by TNF-α. [Conclusions] Inhibiting MCP-1 and IL-1β overexpression in atheroclerosis process may be one of the mechanisms of transhinone IIA. |
| Key words: tanshinone ⅡA MCP-1 IL-1β experimental research |
