| 摘要: |
| [目的]研究中药复方妍婷颗粒对脂多糖(LPS)诱导小鼠脾细胞产生肿瘤坏死因子-α(TNF-α)的影响,初步探讨妍婷颗粒对慢性盆腔炎抗炎、免疫调节的内在机制。[方法]体外培养小鼠脾细胞用LPS刺激,用不同浓度妍婷颗粒含药血清干预,酶联免疫银光法(ELISA)法检测上清液中TNF-ɑ含量,反向逆转录聚合酶链式反应(RT-PCR)法测细胞中TNF-ɑmRNA表达。[结果]1)经LPS诱导后小鼠脾细胞TNF-ɑ分泌量明显升高,TNF-ɑmRNA表达增强。2)小鼠脾细胞TNF-ɑ的分泌量与表达与妍婷颗粒含药血清浓度呈剂量依赖性。[结论]妍婷颗粒具有抑制LPS诱导的小鼠脾细胞产生TNF-ɑ的作用,据此推测妍婷颗粒的抗炎、免疫调节作用机制可能与其抑制脂多糖介导的TNF-ɑ释放有关。 |
| 关键词: 妍婷颗粒 脂多糖 脾细胞 肿瘤坏死因子 |
| DOI：10.11656/j.issn.1672-1519.2009.02.26 |
| 分类号: |
| 基金项目:黑龙江省科技攻关计划项目(GC01C142-02) |
|
| Effect of Yanting Granules on TNF-α secretion of spleen cells induced by lipopolysaccharide in mice |
|
CAI Jing1, QI Bing2, HOU Li-hui2
|
|
1.Yantai Harbor Hospital, Yantai 264000, China;2.Heilongjiang University of TCM, Haerbin 150040, China
|
| Abstract: |
| [Objective] To explore the effect of a compound prescription of Chinese medicine Yanting Granules on TNF-α secretion of spleen cell induced by lipopolysaccharide(LPS) in mice and the immunoregulation of Yanting Granules in treating chronic pelvic inflam-matory disease (CPID). [Methods] TNF-α expression in spleen cells of mice was induced by LPS and spleen cells of mice were treated with medicine-contained serum of Yanting Granules. TNF-α synthesis was detected using ELISA and RT-PCR. [Results] Compared with the normal group,the secretion of TNF-α was significantly increased in LPS group. Medicine-contained serum of different concentrations could reduce the secretion of TNF-α. [Conclusion] The results suggest that the resistance function of Yanting Granules against the in-flammation and immunoregulation may be related to the inhibitory effect of the release of TNF-α secretion. |
| Key words: Yanting Granules LPS spleen cell TNF-α |
