| 摘要: |
| [目的]采用反相高效液相色谱法,建立河北产北败酱中绿原酸的含量测定方法及其指纹图谱。[方法]用Kromasil C18色谱柱,乙腈、水梯度洗脱;流速10mL/min;柱温35℃;检测波长327nm。[结果]建立了高效液相色谱(HPLC)指纹图谱共有模式,并对其进行了相似度比较。[结论]北败酱中绿原酸及其他各成分均得到很好分离,采用HPLC指纹图谱可有效控制北败酱的质量。 |
| 关键词: 北败酱 绿原酸 高效液相色谱 指纹图谱 |
| DOI：10.11656/j.issn.1672-1519.2009.02.28 |
| 分类号: |
| 基金项目:天津市卫生局中医、中西医结合科研课题(07017) |
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| Determination of chlorogenic acid and establishment of fingerprint spectrum of cortex dictamni produced in Hebei Province |
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LIU Jun-hong, LI Di-hua, WU Xiao-xian
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Institute of Acute Abdomen of Traditional Chinese Medicine and Western Medicine, Tianjin 300100, China
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| Abstract: |
| [Objective] To study the determination of chlorogenic acid and establishment of fingerprint spectrum of Cortex Dictamni produced in Hebei province by reversed phased high performance liquid chromatography. [Methods] A Kromasil C18 column was used;the mobile phase was composed of acetonitrile-water with gradient elution. The flow rate was 10 mL/min;The temperature of column was 35℃ and the wavelength of detection was 327 nm. [Results] The mutual mode of HPLC fingerprint was set up,and comparison of similarity was carried out. [Conclusion] Under the selected chromatographic conditions,Chlorogenic acid of Cortex Dictamni and other composi-tion was separated well,and the Quality of Cortex Dictamni can be controlled effectively by HPLC fingerprint. |
| Key words: Cortex Dictamni chlorogenic acid HPLC fingerprint |
