| 摘要: |
| [目的] 建立同时测定连翘药用部位和非药用部位中连翘酯苷A、连翘酯苷B、紫云英苷、连翘苷、牛蒡子苷、槲皮素和牛蒡子苷元7种化学成分的高效液相色谱（HPLC）方法。[方法] 采用反相高效液相色谱法，以Agilent Eclipse Plus C18（4.6 mm×100 mm，1.8 μm）为色谱柱；流动相为乙腈-0.1%甲酸水，梯度洗脱，检测波长为280 nm，体积流量为0.3 mL/min，柱温为30℃。[结果] 连翘酯苷A、连翘酯苷B、紫云英苷、连翘苷、牛蒡子苷、槲皮素和牛蒡子苷元分离与线性关系良好，平均回收率分别为100%、99.1%、104%、104%、98.4%、99.0%、103%，RSD 分别为2.27%、1.59%、1.94%、1.25%、2.97%、2.10%、2.67%.[结论] 该方法分离度好，简便快速，可用于同时检测连翘药用及非药用部位中连翘酯苷A，连翘酯苷B，紫云英苷，连翘苷，牛蒡子苷，槲皮素和牛蒡子苷元的含量，为连翘资源全面质量评价提供依据。 |
| 关键词: 高效液相色谱法 连翘 连翘酯苷A 连翘酯苷B 紫云英苷 连翘苷 |
| DOI：10.11656/j.issn.1672-1519.2014.07.16 |
| 分类号: |
| 基金项目:国家科技基础性工作项目（2007FY110600），高等学校博士学科点专项科研基金项目（20101210120007），国家自然科学基金项目资助（810001632），天津市科技支撑计划重大项目（10ZCADSY12400） |
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| Simultaneous determination of seven components in medicinal and non-medicinal parts of Forsythia suspensa by RP-HPLC |
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LI Jin, WANG Chun-peng, MA Lin, ZHANG Jian, HU Jing, CHANG Yan-xu
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Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
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| Abstract: |
| [Objective] To establish an HPLC method for the simultaneous determination of forsythoside A, forsythoside B, astragalin, forsythin, arctiin, quercetin and arctigenin in Medicinal and non-medicinal parts of Forsythia suspensa. [Methods] The analysis was performed on an Agilent Eclipse Plus C18(4.6×100 mm, 1.8 μm). The mobile phase was composed of acetonitrile and 0.1% formic acid with gradient elution. The detection wavelength was set at 280 nm. The flow rate was 0.3 mL/min at column temperature of 30℃. [Results] Forsythoside A, forsythoside B, astragalin, forsythin, arctiin, quercetin and arctigenin were well separated by HPLC and showed a good linearity ranges. The average recoveries of the seven components were 100%, 99.1%, 104%, 104%, 98.4%, 99.0% and 103% and the RSD values were 2.27%, 1.59%, 1.94%, 1.25%, 2.97%, 2.10% and 2.67%, respectively. [Conclusion] A rapid and simple HPLC method was established to simultaneously determine the contents of the seven components in Forsythia suspense, which provides a scientific basis for the quality control of Forsythia suspense. |
| Key words: HPLC Forsythia suspensa (Thunb.) Vahl forsythoside A forsythoside B astragalin forsythin |
