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| 香柑内酯在不同种属肝微粒体中的代谢差异研究 |
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刘文莉1, 只德贤1,2, 王丽莉1, 刘昌孝1,3, 何新1,4
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1.天津中医药大学中药学院, 天津 301617;2.天津商业大学生物技术与食品科学学院, 天津 300134;3.天津药物研究院释药技术与药代动力学国家重点实验室, 天津 300193;4.广东药科大学中药学院, 广州 510006
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| 摘要: |
| [目的]研究香柑内酯在7种不同肝微粒体中的代谢差异。[方法]采用肝微粒体体外孵育的方法研究香柑内酯在7种不同种属肝微粒体中的代谢差异。使用四级杆飞行时间液质联用（Q-TOF-LC/MS）法检测分析代谢产物。[结果]香柑内酯与7种肝微粒体孵育后共检测出4种不同的代谢产物，通过分析代谢产物的种类及含量，香柑内酯在比格犬和大鼠肝微粒体中的代谢与在人肝微粒的代谢较为相近。[结论]通过研究发现，香柑内酯在比格犬和大鼠肝微粒体中的代谢与在人肝微粒体中的代谢较为接近。 |
| 关键词: 香柑内酯 肝微粒体 代谢产物 种属差异 |
| DOI：10.11656/j.issn.1672-1519.2019.09.21 |
| 分类号:R969.1 |
| 基金项目:国家自然科学基金重点项目（81430096）；天津市科技支撑计划重点项目（16YFZCSY00440）；教育部创新团队发展计划资助项目（IRT_14R41）。 |
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| Study of the differences in bergapten metabolites in liver microsomes of different species and genera |
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LIU Wenli1, ZHI Dexian1,2, WANG Lili1, LIU Changxiao1,3, HE Xin1,4
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1.School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.School of Biotechnology Food Science, Tianjin University of Commerce, Tianjin 300134, China;3.Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China;4.School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China
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| Abstract: |
| [Objective] To study the differences in bergapten metabolites in seven liver microsomes.[Methods] Liver microsomes were incubated in vitro to study the metabolic differences in liver microsomes of seven species of animals. The metabolites of bergapten incubated in different species of liver microsomes were detected by Q-TOF-LC/MS.[Results] After incubation of bergapten with liver microsomes of seven species,four kinds of metabolites were detected. By comparing and analyzing the types and quantities of metabolites,the metabolism of bergapten in liver microsomes of beagle dog and rat are similar to that in human liver microsomes.[Conclusion] It was found that the metabolism of bergapten in liver microsomes of beagle dog and rat were similar to that in human liver microsomes. |
| Key words: bergapten liver microsome metabolite species difference |
