| 摘要: |
| [目的] 探赜宣肺清络方治疗肺炎支原体肺炎（MPP）痉咳的效果及作用机制。[方法] 将60只Wistar大鼠随机分为空白组、支原体肺炎组、支原体肺炎痉咳、中药组、西药组、中西医结合组，每组10只。除空白组外，支原体肺炎组进行支原体肺炎培养液（1×107 CCU/mL）滴鼻建立MPP肺炎模型，其余各组大鼠采用支原体肺炎培养液（1×107 CCU/mL）滴鼻联合烟熏法、辣椒素溶液雾化法建立支原体肺炎感染后痉咳大鼠模型。造模成功后，分别给予中药组14 g/（kg·d）、西药组63 mg/（kg·d）、中西医结合组14 g/（kg·d）+63 mg/（kg·d）以相应剂量浓度药液灌胃给药，连续治疗7 d。空白组、支原体肺炎组、支原体肺炎痉咳组采用0.9%氯化钠溶液灌胃对照。末次给药24 h后，采用酶联免疫吸附测定（ELISA）技术检测大鼠血清中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）蛋白表达水平，运用蛋白免疫印迹法（Western blot）对大鼠肺组织中Toll样受体4（TLR4）、髓样分化因子88（MyD88）、核因子-κB（NF-κB）信号通路相关蛋白表达水平，实时荧光定量聚合酶链反应（Real-time PCR）检测IL-6、IL-8、TNF-α以及TLR4、MyD88、NF-κB mRNA表达水平。[结果] 与空白组相比，支原体肺炎组、支原体肺炎痉咳组大鼠血清IL-6、IL-8、TNF-α含量明显上升（P<0.05或P<0.001）；肺脏组织TLR4、MyD88、NF-κB蛋白表达水平显著上调（P<0.01或P<0.001）；肺脏组织TLR4、MyD88、NF-κB、IL-6、TNF-α、IL-8mRNA表达水平显著上调（P<0.01或P<0.001）。经治疗后，与支原体肺炎痉咳组相比，用药组大鼠血清IL-6、IL-8、TNF-α水平显著降低（P<0.01或P<0.001），肺脏组织TLR4、MyD88、NF-κB蛋白表达显著降低（P<0.01或P<0.001），TLR4、MyD88、NF-κB-p65、IL-6、IL-8、TNF-α mRNA表达水平显著降低（P<0.001）。[结论] 宣肺清络方可以通过调控TLR4/MyD88/NF-κB信号通路，抑制MPP引起的氧化应激，降低气道高反应，达到改善肺组织微循环障碍，从而发挥治疗支原体肺炎痉咳的作用。 |
| 关键词: 宣肺清络方 TLR4/MyD88/NF-κB通路 痉咳 |
| DOI：10.11656/j.issn.1672-1519.2026.07.12 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目（8200152470）。 |
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| Exploration on the mechanism of Xuanfei Qingluo Formula on mycoplasma pneumoniae-induced paroxysmal cough model rats based on the TLR4/MyD88/NF-κB pathway |
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LI Yadong1, ZHANG Qi1, LIU Weiwei1, SUN Yuechen2
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1.The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China;2.Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China
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| Abstract: |
| [Objective] To explore the effect and mechanism of Xuanfei Qingluo Formula in the treatment of paroxysmal cough caused by Mycoplasma pneumoniae pneumonia（MPP）. [Methods] Sixty Wistar rats were randomly divided into a blank group，a mycoplasma pneumonia group，a mycoplasma pneumonia with paroxysmal cough group，a traditional Chinese medicine group，a Western medicine group，and a combined traditional Chinese and Western medicine group，with 10 rats in each group. Except for the blank group，the mycoplasma pneumonia group was intranasally instilled with mycoplasma pneumonia culture fluid（1×107 CCU/mL）to establish a MPP pneumonia model，while the other groups were intranasally instilled with mycoplasma pneumonia culture fluid（1×107 CCU/mL）and exposed to smoke and capsaicin solution nebulization to establish a mycoplasma pneumonia-induced paroxysmal cough rat mode. After successful modeling，the traditional Chinese medicine group was given 14 g/（kg·d），the Western medicine group was given 63 mg/（kg·d）， and the combined traditional Chinese and Western medicine group was given 14 g/（kg·d）+ 63 mg/（kg·d）of the corresponding drug solution by gavage for 7 consecutive days. The blank group，the mycoplasma pneumonia group，and the mycoplasma pneumonia with paroxysmal cough group were given 0.9% sodium chloride solution by gavage as a control. 24 hours after the last administration，the levels of interleukin-6（IL-6），interleukin-8（IL-8），and tumor necrosis factor-α（TNF-α）in the rat serum were detected by enzyme-linked immunosorbent assay（ELISA）. The expression levels of Toll-like receptor 4（TLR4），myeloid differentiation factor 88（MyD88）， and nuclear factor-κB（NF-κB）in the lung tissue of rats were detected by Western blot，and the mRNA expression levels of IL-6，IL-8， TNF-α，TLR4，MyD88，and NF-κB were detected by real-time fluorescence quantitative polymerase chain reaction（Real-time PCR）. [Results] Compared with the blank group，the levels of IL-6，IL-8 and TNF-α in the serum of rats in the mycoplasma pneumonia group and the mycoplasma pneumonia with paroxysmal cough group were significantly increased（P<0.05 or P<0.001）；the expression levels of TLR4，MyD88 and NF-κB proteins in lung tissue were significantly upregulated（P<0.001 or P<0.01）；the expression levels of TLR4，MyD88，NF-κB，IL-6，TNF-α and IL-8 mRNA in lung tissue were significantly upregulated（P<0.001 or P<0.01）. After treatment，compared with the mycoplasma pneumonia with paroxysmal cough group，the levels of IL-6，IL-8 and TNF-α in the serum of rats in the traditional Chinese medicine group，the Western medicine group and the integrated traditional Chinese and Western medicine group were significantly decreased（P<0.01 or P<0.001）；the expression levels of TLR4，MyD88 and NF-κB proteins in lung tissue were significantly decreased（P<0.01 or P<0.001）；the expression levels of TLR4，MyD88，NF-κB-p65，IL-6，IL-8 and TNF-α mRNA in lung tissue were significantly decreased（P<0.001）. [Conclusion] The Xuanfei Qingluo Formula can exert therapeutic effects on mycoplasma pneumonia-induced spasmodic cough by regulating the TLR4/MyD88/NF-κB signaling pathway，inhibiting the oxidative stress caused by MPP，and reducing airway hyperresponsiveness，thereby improving the microcirculation disorder of lung tissue. |
| Key words: Xuanfei Qingluo Formula TLR4/MyD88/NF-κB pathway paroxysmal cough |
