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| Expression of P450arom,CYP19 and TGF-β1 in the testis of infertile rats with kidney-yang deficiency |
| Hits 2312 Download times 2350 Received:December 30, 2009 |
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| DOI
10.11656/j.issn.1672-1519.2010.03.30 |
| Key Words
kidney-yang deficiency;testis;rat;P450arom;CYP19;TGF-β1 |
| Author Name | Affiliation | | LI Rui | Department of Traditional Chinese of Xijing Hospital, Xi’an 710032, China | | LIU Man-li | Department of Traditional Chinese of Xijing Hospital, Xi’an 710032, China | | TIAN Xin | Department of Traditional Chinese of Xijing Hospital, Xi’an 710032, China | | 谢娟 | Department of Traditional Chinese of Xijing Hospital, Xi’an 710032, China | | 周大伟 | Department of Traditional Chinese of Xijing Hospital, Xi’an 710032, China | | 李臻 | The Forth military Medical University, Xi’an 710032, China | | 马静 | Department of Traditional Chinese of Xijing Hospital, Xi’an 710032, China |
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| Abstract
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| [Objection] To study the expression of P450arom,CYP19 and TGF-β1 in the testis of infertile rats with adenine-modeled kidney-yang deficiency and the pathologicalmechanian of infertility with kidney-yang deficiency.[Methods] Twenty-three month male SD rats were divided randomly into four groups with five in each group(normal control,model,Ⅰ,Ⅱ and Ⅲ).The experimental rats had been fed with adenine [100,150,200(mg/kg·d)] in 30 days and the expression levels of P450arom,CYP19,TGF-β1 were measured with immunohistochemical SABC method.[Results] CYP19 was expressed in cell of Leydig,dolicho-nematoblast in rats’testis;P450arom and TGF-β1 were positive expressed in cytoplasm of Leydig’s cell;with their positive immuno-responsive substance locating in the cytoplasm.Compared with the normal control,TGF-β1 expression was higher in kidney-yang deficiency group(P<0.05);CYP19 and P450arom expression were higher in the kidney-yang deficiney group(P<0.05).Among the three model groups,the expression of TGF-β1,P450arom,CYP19 had no significant deviation(P>0.05).[Conclusion] The abnormality expression P450arom,CYP19,TGF-β1 in testis was possible with infertility with kidney-yang deficiency,for further approach P450 arom,CYP19,TGF-β1in infertility rats with kidney-yang deficiency gonepoiesis and correlation provided preliminary experimental evidence. |
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