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| Study on PI3K/Akt messenger passage and apoptosis controlling in PESV-preconditioned K562 cells |
| Hits 2531 Download times 2379 Received:February 28, 2012 |
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| DOI
10.11656/j.issn.1672-1519.2012.03.24 |
| Key Words
PESV;K562 cell lines;apoptosis;PI3K;Akt;Bcl-2;Bad |
| Author Name | Affiliation | | ZHANG Lei | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China | | YANG Wen-hua | The First Hospital Affiliated of Tianjin University of TCM, Tianjin 300193, China | | YU Wen-jun | Tianjin University of TCM, Tianjin 300193, China | | HAO Zheng | Tianjin University of TCM, Tianjin 300193, China | | ZHANG Jia | Tianjin University of TCM, Tianjin 300193, China |
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| Abstract
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| [Objective] To investigate the effect of PESV on the expression of massage protein PI3K / Akt, apoptosis regulators Bcl-2 and Bad expression in K562 cells. [Methods] In vitro cultured K562 cells preconditioned with PESV for different times were determined for cell apoptosis rate with cytoflowmeter, the level of PI3K and p-AKT protein with Western blot and Bcl-2, Bad mRNA level by realtime fluorescence quantitative RT-PCR. [Results] Compared with the control group, the apoptosis rate of PESV treated K562 cells was significantly increaseds, the expression of PI3K and p-Akt and anti-apoptosis-related gene Bcl-2 mRNA was decreased; the expression of pro-apoptotic gene Bad mRNA was increased. [Conclusion] PESV may regulate the expression of Bcl-2 and Bad, inhibit the proliferation of K562 cells and promote their apoptosis by reducing the expression of PI3K and Akt signaling protein. |
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