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| Optimization of the extraction technique of Codonopsis tangshen Dliv. polysaccharide using response surface methodoloy |
| Hits 2203 Download times 2702 Received:February 03, 2015 |
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| DOI
10.11656/j.issn.1672-1519.2015.07.13 |
| Key Words
Codonopsis tangshen Oliv.;polysaccharide;RSM;extraction technique |
| Author Name | Affiliation | E-mail | | WU Xin-liang | Department of Pharmacy, Tianjin Baodi Hospital, Tianjin 301800, China | | | WANG Hong-jun | Liaoning Medical University, Jinzhou 121001, China | lnwhj2012@163.com | | BAI Zi-xia | Department of Pharmacy, Tianjin Baodi Hospital, Tianjin 301800, China | | | LONG Yuan-tao | Liaoning Medical University, Jinzhou 121001, China | |
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| Abstract
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| [Objective] To optimize the extraction conditions of polysaccharide in Codonopsis tangshen Oliv. using response surface methodology (RSM). [Methods] On the basis of single factor test, extracting temperature, extracting times and extracting time were selected as response factors; the yield of polysaccharide was used as response value. A three-factor-three-level experiment design was developed. RSM was employed to analyze the results of experiments. [Results] The optimum extraction conditions of polysaccharide were as follows: extracting temperature was 80 ℃, extracting times was 5 times, and extracting time was 5 h. The yield of polysaccharide was 22.31%. [Conclusion] RSM employed in optimization of the extraction conditions is feasible. |
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