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| Study of puerarin induce apoptosis and autophagy in human liver cancer HepG2 cells and its molecular mechanism |
| Hits 1243 Download times 739 Received:June 28, 2021 |
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| DOI
10.11656/j.issn.1672-1519.2021.11.22 |
| Key Words
puerarin;liver cancer;apoptosis;autophagy;Akt/mTOR/Beclin1 pathway |
| Author Name | Affiliation | | HU Yali | The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China | | YANG Jie | The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China | | PANG Qianqian | The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China |
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| Abstract
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| [Objective] To study the effects of puerarin induce apoptosis and autophagy in human liver cancer HepG2 cells and its molecular mechanism. [Methods] The DMSO (blank control group),puerarin (50,100,200 μg/mL) and cisplatin 20 μg/mL were used to interfere with human liver cancer HepG2 cells in logarithmic growth respectively. 48h later,the proliferation inhibition rate of HepG2 cells was detected by CCK-8 method,the apoptosis level was detected by Annexin V-FITC/PI double staining;the autophagy of cells was observed by acridine orange staining;the expression of Akt,p-Akt,Cleaved Caspase-3,bcl-2,Bax,p-mTOR,Beclin1,LC3 proteins were detected by Western blot. [Results] Compared with blank group,the proliferation inhibition rate and apoptosis rate of puerarin 100 μg/mL group. Puerarine 200 μg/mL group and cisplatin 20 μg/mL group were increased (P<0.01),the number of autophagolysosomes was significantly increased (P<0.01);the expression of p-Akt,p-mTOR,bcl-2 were down-regulated and the expression of Cleaved Caspase-3,Bax,Beclin1,LC3-Ⅰ,LC3-Ⅱ were up-regulated (P<0.05 or P<0.01),the Akt phosphorylation ratio (p-Akt/Akt) was decreased and the Bax/bcl-2,LC3-Ⅱ/LC3-Ⅰ ratio were increased (P<0.01). Compared with Cisplatin 20 μg/mL group,the proliferation inhibition rate and apoptosis rate of puerarin 200 μg/mL group were increased(P<0.01 or P<0.01),the number of autophagolysosomes was significantly increased (P<0.05);the expression of p-Akt,p-mTOR were down-regulated and the expression of Cleaved Caspase-3,Bax,Beclin1,LC3-Ⅱ were up-regulated (P<0.05 or P<0.01),the Akt phosphorylation ratio was decreased and the Bax/bcl-2,LC3-Ⅱ/LC3-Ⅰratio were increased (P<0.01). [Conclusion] Puerarin can induce apoptosis and autophagy in human liver cancer HepG2 cells,which has a dose-dependent effect;inhibiting Akt/mTOR signaling pathway to induce pro-apoptosis,autophagy-related protein expression and activation may be its important molecular mechanism. |
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