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| The effect of curcumin regulating the Notch1/Hes1/Prdx1 pathway on IL-1β-induced inflammatory injury of chondrocytes |
| Hits 193 Download times 42 Received:January 12, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.06.12 |
| Key Words
curcumin;Notch 1;hairy and enhancer of split homolog 1;peroxiredoxin 1;interleukin-1β;inflammatory injury |
| Author Name | Affiliation | E-mail | | LU Zhaoping | Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China | | | HE Jiandong | Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China | | | TAN Zhichao | Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China | | | XIE Shangneng | Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China | xsnfine@163.com |
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| Abstract
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| [Objective] To investigate the effect of curcumin（Cur）regulating the Notch1/hairy and enhancer of split homolog 1（Hes1）/ peroxiredoxin 1（Prdx1）pathway on inflammatory injury of chondrocytes induced by interleukin-1 β（IL-1β）. [Methods] C28/I2 cells were randomly separated into Ctrl group（normal culture），IL-1β group（10 ng/mL IL-1β），low-dose Cur （L-CUR），medium-dose Cur （M- Cur），and high-dose Cur （H-Cur）groups（IL-1β induced+10，20，40 μmol/L Cur），and Cur+Jagged 1 group（IL-1β induced+40 μmol/L Cur+0.5 μg/mL Notch activator Jagged 1）. The proliferation of C28/I2 cells was detected by MTS assay and 5-Ethynyl-2'-deoxyuridine （EdU）staining. The apoptosis of C28/I2 cells was detected by flow cytometry. The 2'，7'-dichlorofluorescein diacetate（DCFH-DA） fluorescent probe was used to detect the level of reactive oxygen species（ROS）in C28/I2 cells. Immunofluorescence was used to detect the expressions of type Ⅱ collagen（ColⅡ）and Aggrecan in C28/I2 cells. Enzyme-linked immunosorbent assay（ELISA）kits were used to detect the expression of interleukin（IL）-6，IL-8，tumor necrosis factor-α（TNF-α），matrix metalloproteinase（MMP）-3，and MMP-13 in C28 / I2 cells. Western blot was used to detect the protein expression of proliferating cell nuclear antigen（Ki-67）， minichromosome maintenance complex component 7（MCM）7，Notch1，Hes1，Prdx1，BCL2 antagonist（Bak），and survival protein （survivin）in C28 /I2 cells. [Results] The OD490 value，EdU positive cell rate，ColⅡ fluorescence intensity，Aggrecan fluorescence intensity，Ki-67，MCM7 and survivin in the IL-1β group were lower than those in the Ctrl group，while the apoptosis rate，ROS fluorescence，Bak，MMP-3，MMP-13，IL-6，IL-8，TNF-α，Notch1，Hes1 and Prdx1 were higher than those in the Ctrl group（P<0.05）. The OD 490 value，EdU positive cell rate，ColⅡ fluorescence intensity，Aggrecan fluorescence intensity，Ki-67，MCM7 and survivin in the L-Cur group，M-Cur group，H-Cur group were higher than those in the IL-1β group，while the apoptosis rate，ROS fluorescence， Bak，MMP-3，MMP-13，IL-6，IL-8，TNF-α，Notch1，Hes1 and Prdx1 were lower than those in the IL-1β group（P<0.05）. The OD490 value，EdU positive cell rate，ColⅡ fluorescence intensity，Aggrecan fluorescence intensity，Ki-67，MCM7 and survivin in the Cur+ Jagged 1 group were lower than those in the H-Cur group，while the apoptosis rate，ROS fluorescence，Bak，MMP-3，MMP-13，IL-6，IL-8， TNF-α，Notch1，Hes1 and Prdx1 were higher than those in the H-Cur group（P<0.05）. [Conclusion] Cur alleviates IL-1β-induced inflammatory injury of chondrocytes by inhibiting the Notch1/Hes1/Prdx1 pathway. |
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