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| Exploring the neuroprotective effect of Huangzhu Qingnao Granules on rats with cerebral ischemia-reperfusion injury based on NLRP3 inflammasome-mediated pyroptosis |
| Hits 197 Download times 59 Received:January 25, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.06.14 |
| Key Words
Huangzhu Qingnao Granule;cerebral ischemia-reperfusion injury;CIRI;MCAO/R;NLRP3;pyroptosis |
| Author Name | Affiliation | E-mail | | XIONG Peng | Department of Geriatrics, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
The First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang 712000, China | | | XUE Ruiwen | Department of Geriatrics, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
The First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang 712000, China | xueruiwen78@163.com | | WANG Dengkun | Department of Geriatrics, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China | | | NING Changmei | The First Clinical Medical College, Shaanxi University of Chinese Medicine, Xianyang 712000, China | |
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| Abstract
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| [Objective] To investigate the neuroprotective effect of Huangzhu Qingnao Granules on rats with middle cerebral artery occlusion and reperfusion（MCAO / R）model，and its influence on the NLRP3 inflammasome-mediated pyroptosis pathway. [Methods] Forty male Sprague-Dawley（SD）rats were randomly divided into sham operation group，model group，butylphthalide group， and Huangzhu Qingnao Granules group. The MCAO/R model was used to simulate cerebral ischemia-reperfusion injury（CIRI），and corresponding drugs were administered by gavage for 4 weeks after successful modeling. Neurological deficits were evaluated by Zea-longa scoring；cerebral infarction area was determined by TTC staining；pathological morphology of brain tissue was observed by HE staining；levels of IL- 18，IL-1β，and SAA in brain tissue were detected by ELISA；cell apoptosis was observed by TUNEL staining；positive expression of IBA-1 and NLRP3 in brain tissue was detected by immunofluorescence double staining；relative expressions of NLRP3，GSDMD，GSDMD-N，Caspase-1，Cleaved-Caspase-1，and GFAP in brain tissue were detected by Western Blot. [Results] Compared with the model group，the Zea-longa score and cerebral infarction area in the Huangzhu Qingnao Granules group were significantly reduced（P<0.05 or P<0.01），and pathological damage of brain tissue was alleviated. The levels of IL-18，IL-1β，and SAA in brain tissue were significantly decreased（P<0.01）. The positive expressions of IBA-1 and NLRP3 proteins were significantly reduced（P<0.01），and the relative expressions of NLRP3，GSDMD-N，Cleaved-Caspase-1，and GFAP proteins were significantly decreased（P<0.01）. [Conclusion] Huangzhu Qingnao Granules may treat CIRI by inhibiting NLRP3 inflammasome-mediated pyroptosis，reducing the release of pro-inflammatory neurofactors，and alleviating neuropathological damage and neuromotor function deficits in MCAO/R rats. |
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