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Jianpi Huayu Decoction inhibits EMT in hepatoma cells via regulating the CD44/ERK pathway
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DOI   10.11656/j.issn.1672-1519.2026.06.16
Key Words   hepatocellular carcinoma cell;Jianpi Huayu Decoction;EMT;CD44/ERK
Author NameAffiliationE-mail
YIN Bing The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		 
FENG Kunliang The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		 
WANG Ziyu The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		 
FANG Chongkai The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		 
LUO Rui The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		 
LAI Yongheng The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		 
ZHONG Chong The First Clinical Medical School of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China
State Key Laboratory of Traditional Chinese Medicine Syndrome/The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China 		zhongchong1732@gzucm.edu.cn 
Abstract
    [Objective] To study the inhibiting effect of Jianpi Huayu Decoction（JHD）in epithelial-mesenchymal transition（EMT）of hepatoma carcinoma cell，and to explore the possible mechanism behind it. [Methods] Culture MHCC97H cells in vitro，and divide the MHCC97H cells into three groups according to the stimulations（control group，10% of the drug-containing serum group，20% of the drug-containing serum group）.The cell migration and invasion abilities was evaluated by scratch test and Transwell invasion test， and Real-time PCR and Western Blot were used to detect the expression levels of genes and proteins related to the EMT pathway. [Results]Compared with control group，the migration and invasion ability of the drug-containing serum groups were significantly decreased. As well as，the mRNA and protein expression level of Cluster of differentiation 44（CD44），Extracellular regulated kinase （ERK），zinc finger protein transcription factor（Snail），Vimentin and N-cadherin were significantly decreased，while the expression of E-cadherin was increased. [Conclusion] JHD may inhibits EMT，migration，and invasion of hepatocellular carcinoma MHCC97H cells through inhibiting the activation of the CD44/ERK pathway.

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