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| Study on the synergistic mechanism of Lung-nourishing and Blood-activating Formula combined with amniotic mesenchymal stem cell transplantation in treating hypoxic pulmonary hypertension in rats |
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| DOI
10.11656/j.issn.1672-1519.2026.08.11 |
| Key Words
pulmonary hypertension;Lung-nourishing and Blood-activating Formula;mesenchymal stem cells;endothelial-mesenchymal transition |
| Author Name | Affiliation | E-mail | | YANG Jingkun | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIANG Nan | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | FENG Jihong | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | ZHANG Lina | NMPA Key Laboratory for Quality Control of Cell and Gene Therapy Medicine Products, Northeast Normal University, Changchun 130024, China | | | XING Shaoliang | NMPA Key Laboratory for Quality Control of Cell and Gene Therapy Medicine Products, Northeast Normal University, Changchun 130024, China | | | ZHANG Shuo | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China
Tianjin Binhai New Area Hospital of Traditional Chinese Medicine (The Fourth Affiliated Hospital of Tianjin University of Traditional Chinese Medicine), Tianjin 300450, China | sureares@126.com |
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| Abstract
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| Objective This study investigated the synergistic therapeutic effects and explored the underlying mechanisms of the Lung-nourishing and Blood-activating Formula combined with amniotic mesenchymal stem cell (MSC) transplantation in rat models of hypoxic pulmonary arterial hypertension. Methods A hypoxic pulmonary arterial hypertension rat model was established, and 24 successfully modeled rats were divided into four groups: the model group, the traditional Chinese medicine (TCM) group, the stem cell group, and the combined therapy group. The groups received oral administration of the Lung-nourishing and Blood-activating Formula, tail vein injection of MSC, or combined intervention, respectively. Results Results demonstrated that the combined therapy group exhibited greater reductions in right ventricular hypertrophy index (RVHI); improved percentage of pulmonary artery thickness (WT%) and percentage of pulmonary artery area (WA%); suppressed the expression of endothelium-mesenchymal transition (EndoMT)-related markers (α-SMA, Snail, and MMP9); upregulated endothelial cell markers (VE-cadherin); reduced inflammatory factor levels (VEGF, TNF-α, and IL-6) compared with the monotherapy groups (P < 0.05). Mechanistic studies revealed that the combination therapy effectively mitigated pulmonary arterial hypertension progression by inhibiting the EndoMT process, improving vascular endothelial barrier function, and synergistically regulating inflammatory responses and vascular remodeling. Conclusion This study provides experimental evidence for the integrated traditional Chinese and Western medicine strategy in treating pulmonary arterial hypertension, confirming the significant synergistic effect of combining the Lung-nourishing and Blood-activating Formula with MSC. |
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