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| Explore the effect and mechanism of Fuzheng Quxie method in inhibiting airway mucus hypersecretion in COPD rats based on the TACE-EGFR signaling pathway |
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| DOI
10.11656/j.issn.1672-1519.2026.08.12 |
| Key Words
chronic obstructive pulmonary disease;airway mucus hypersecretion;Bufei Granules;TACE-EGFR signaling pathway |
| Author Name | Affiliation | E-mail | | LI Yunhui | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | PENG Xin | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | YANG Kun | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | ZHANG Yihao | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | LUAN Zheyu | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | 623084194@qq.com |
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| Abstract
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| Objective To establish a stable-phase chronic obstructive pulmonary disease (COPD) rat model and investigate the mechanism of Bufei Granules (BFKL), a Chinese herbal medicine for reinforcing healthy qi and eliminating pathogens, in inhibiting airway mucus hypersecretion and reducing bacterial load via the TACE-EGFR signaling pathway. Methods The 40 Wistar rats were randomly divided into five groups: blank control, model, acetylcysteine, reinforcing healthy qi herbal group (FZ), and reinforcing healthy qi-eliminating pathogens herbal group (FZQX). Except for the blank control group, all rats were subjected to cigarette smoke exposure combined with slow intratracheal instillation of Streptococcus pneumoniae agar suspension to establish an acute exacerbation of COPD (AECOPD) model. After stabilization, a 1-month drug intervention was administered. Pathological changes in lung tissue were analyzed via HE staining, bacterial load was assessed via colony counting, serum and bronchoalveolar lavage fluid (BALF) levels of TACE, TGF-α, and MUC5AC were measured using ELISA, and TACE/EGFR mRNA and protein expression were detected via RT-PCR and Western blot. Results Compared with the blank control group, the model group exhibited significant bronchial structural damage, dense bacterial colony growth in lung homogenate cultures, elevated serum and BALF levels of TGF-α, TACE, and MUC5AC (P < 0.01), and increased TACE/EGFR mRNA and protein expression in lung tissue (P < 0.01). Compared with the model group, the FZQX herbal group showed alleviated bronchial damage, reduced submucosal inflammatory cell infiltration, fewer bacterial colonies, decreased TGF-α, TACE, and MUC5AC levels (P < 0.05), and downregulated TACE/EGFR mRNA and protein expression (P < 0.05). Conclusion Bufei Granules may inhibit airway mucus hypersecretion and reduce bacterial load by modulating the TACE-EGFR signaling pathway, demonstrating comprehensive therapeutic effects by reinforcing healthy qi and eliminating pathogens. |
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