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| Analysis of active components of Paeoniae Radix Alba to reduce hepatotoxicity of Tripterygium wilfordii based on spectrum-effect relationship |
| Hits 2 Download times 0 Received:April 28, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.09.09 |
| Key Words
Paeoniae Radix Alba;Tripterygium wilfordii;spectrum-effect relationship;hepatotoxicity;active component;UPLC-Q-TOF-MS |
| Author Name | Affiliation | E-mail | | MA Mengyao | School of Chinese Meateria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIANG Jin | School of Chinese Meateria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Wenwen | School of Chinese Meateria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Xiankuan | School of Chinese Meateria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Jinlei | The Sixth Traditional Chinese Medicine Factory of Jinyao Darentang Group, Tianjin 300000, China | wjl6zy@163.com | | WANG Lili | School of Chinese Meateria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wll1980@126.com |
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| Abstract
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| [Objective] Based on the spectrum-effect relationship between the fingerprint of Paeoniae Radix Alba and its efficacy in attenuating the hepatotoxicity of Tripterygium wilfordii ，this study aimed to screen the key active components in Paeoniae Radix Alba responsible for reducing Tripterygium wilfordii -induced liver damage，thereby providing a basis for elucidating the material foundation of its detoxification effect. [Methods] Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry（UPLC-Q-TOF-MS）was employed to establish fingerprints for 20 batches of Paeoniae Radix Alba samples and identify common peaks. The hepatoprotective effect was evaluated using the survival rate of HepG2 cells as the pharmacodynamic index. Grey Relational Analysis（GRA），Principal Component Analysis（PCA），and Orthogonal Partial Least Squares-Discriminant Analysis（OPLS- DA）were comprehensively applied to screen potential active components. For the screened active components，multi-dimensional pharmacodynamic verification was further conducted through cell viability assays，morphological observation，and detection of alanine transaminase（ALT）and aspartate transaminase（AST）levels in the supernatant. [Results] The UPLC- Q-TOF-MS fingerprint was successfully established，with 17 common peaks calibrated and the corresponding components identified. Comprehensive analysis by GRA，PCA，and OPLS-DA revealed that gallic acid，oxypaeoniflorin，albiflorin，paeoniflorin，and benzoylpaeoniflorin were the key active components in Paeoniae Radix Alba for attenuating the hepatotoxicity of Tripterygium wilfordii . Cell experiments confirmed that all five compounds could significantly increase the survival rate of HepG2 cells induced by the main hepatotoxic component of Tripterygium wilfordii triptolide（TP），improve cell morphology，and reduce the levels of ALT and AST in the cell supernatant. [Conclusion] By integrating spectrum- effect relationship analysis with multi- dimensional pharmacodynamic verification，this study identified five active components from Paeoniae Radix Alba that attenuate the hepatotoxicity of Tripterygium wilfordii ，providing a scientific basis for elucidating the material foundation of the detoxification effect of Paeoniae Radix Alba in combination therapy and its clinical application. |
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