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| Mechanism of Bagui Tianlong Powder in ameliorating vascular cognitive impairment by regulating microglial M1/M2 polarization |
| Hits 137 Download times 69 Received:July 07, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.10.10 |
| Key Words
Bagui Tianlong Powder;vascular cognitive impairment;microglia;cell polarization;neuroinflammation |
| Author Name | Affiliation | E-mail | | CHEN Jie | Guangxi University of Chinese Medicine, Nanning 530200, China
Guangxi Key Laboratory of Chinese Medicine Foundation Research, Nanning 530200, China | | | CHEN Yu | Guangxi University of Chinese Medicine, Nanning 530200, China | | | LIU Yiqun | Guangxi University of Chinese Medicine, Nanning 530200, China | | | WEI Ronghuang | Guangxi University of Chinese Medicine, Nanning 530200, China | | | HAO Erwei | Guangxi University of Chinese Medicine, Nanning 530200, China | | | FU Lei | Guangxi University of Chinese Medicine, Nanning 530200, China
Guangxi Key Laboratory of Chinese Medicine Foundation Research, Nanning 530200, China
Guangxi Universities Engineering Research Center for Innovative Product Development of Regional Priority Diseases, Nanning 530200, China | | | YAO Chun | Guangxi University of Chinese Medicine, Nanning 530200, China
Guangxi Key Laboratory of Chinese Medicine Foundation Research, Nanning 530200, China
Guangxi Universities Engineering Research Center for Innovative Product Development of Regional Priority Diseases, Nanning 530200, China | yaoc@gxtcmu.edu.cn |
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| Abstract
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| [Objective] To investigate the mechanism of Bagui Tianlong Powder（BGTLP）in improving vascular cognitive impairment （VCI）based on microglial M1/M2 polarization. [Methods] A VCI rat model was established by bilateral common carotid artery occlusion（2VO）. Rats were randomly divided into model group，Bagui Tianlong group，donepezil group，and sham operation group. After 4 weeks of drug intervention，spontaneous locomotor activity was evaluated by open field test，and learning and memory function was assessed by Morris water maze. Cerebral blood flow perfusion was detected by laser speckle contrast imaging. Hippocampal pathological morphology was observed by hematoxylin-eosin（HE）and Nissl staining. Serum biochemical parameters were measured to evaluate liver and kidney functions. Levels of interleukin-1β（IL-1β），interleukin-6（IL-6），and tumor necrosis factor-α（TNF-α）in serum and brain tissue were detected by enzyme-linked immunosorbent assay（ELISA）. The mRNA expression of microglial polarization markers ionized calcium-binding adapter molecule 1（IBA1），inducible nitric oxide synthase（iNOS），arginase-1（ARG1）， and inflammatory cytokines in hippocampal tissue was detected by quantitative real-time PCR（qRT-PCR）. The protein expression of IBA1，iNOS，and ARG1 in the hippocampus was detected by immunofluorescence staining. [Results] Compared with the model group， the BGTLP group showed increased total distance，average speed，central time，and central distance in the open field test（P<0.05）； significantly shortened escape latency（P<0.01）；increased platform crossing times，target quadrant residence time，target quadrant time ratio and distance ratio（P<0.05）；improved cerebral blood flow perfusion（P<0.01）；alleviated hippocampal damage and Nissl body loss，with no liver or kidney injury. The levels of IL-1β，IL-6 and TNF-α were decreased（P<0.01）. The mRNA expressions of IBA1，iNOS and inflammatory factors were down-regulated，while ARG1 mRNA expression was up-regulated（P<0.05）. Immunofluorescence showed decreased expression of IBA1+ and iNOS+，increased expression of ARG1（+ P<0.05）and decreased iNOS+/ARG1+ratio（P<0.01）. [Conclusion] BGTLP may improve cognitive function in VCI rats by regulating microglial M1/M2 polarization homeostasis and alleviating neuroinflammatory responses. |
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