| 摘要: |
| [目的] 基于白芍指纹图谱与减轻雷公藤肝毒性药效之间的谱效关系，筛选白芍中减轻雷公藤肝毒性的主要活性成分，为阐明白芍减毒作用的物质基础提供依据。[方法] 采用UPLC-Q-TOF-MS法建立20批白芍样品的指纹图谱，确定共有峰。以HepG2细胞存活率为药效指标，评价白芍的减毒作用。综合运用灰色关联度分析（GRA）、主成分分析（PCA）及正交偏最小二乘判别分析（OPLS-DA）筛选白芍潜在的减毒活性成分。对筛选出的活性成分，进一步通过细胞存活率测定、细胞形态观察及上清液中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）含量检测进行多维度药效验证。[结果] 成功建立了白芍样品的UPLC-Q-TOF-MS指纹图谱，标定17个共有峰，并对成分进行鉴定。综合GRA、PCA和OPLS-DA分析，筛选出没食子酸、氧化芍药苷、芍药内酯苷、芍药苷和苯甲酰芍药苷为白芍减轻雷公藤肝毒性的关键活性成分。细胞实验证实，上述5种成分均能显著提升雷公藤主要肝毒性成分雷公藤甲素（TP）诱导的HepG2细胞存活率，改善细胞形态，并降低细胞上清液中ALT和AST的含量。[结论] 该研究通过整合谱效关系分析与多维度药效验证，从白芍中筛选出5个减轻雷公藤肝毒性的活性成分，为阐明白芍联用减毒的物质基础及临床应用提供了科学依据。 |
| 关键词: 白芍 雷公藤 谱效关系 肝毒性 活性成分 UPLC-Q-TOF-MS |
| DOI：10.11656/j.issn.1672-1519.2026.09.09 |
| 分类号:R285.5 |
| 基金项目:天津市卫生健康委员会中西医结合科研课题（2023076）。 |
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| Analysis of active components of Paeoniae Radix Alba to reduce hepatotoxicity of Tripterygium wilfordii based on spectrum-effect relationship |
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MA Mengyao1, JIANG Jin1, LI Wenwen1, LI Xiankuan1, WANG Jinlei2, WANG Lili1
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1.School of Chinese Meateria Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.The Sixth Traditional Chinese Medicine Factory of Jinyao Darentang Group, Tianjin 300000, China
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| Abstract: |
| [Objective] Based on the spectrum-effect relationship between the fingerprint of Paeoniae Radix Alba and its efficacy in attenuating the hepatotoxicity of Tripterygium wilfordii ，this study aimed to screen the key active components in Paeoniae Radix Alba responsible for reducing Tripterygium wilfordii -induced liver damage，thereby providing a basis for elucidating the material foundation of its detoxification effect. [Methods] Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry（UPLC-Q-TOF-MS）was employed to establish fingerprints for 20 batches of Paeoniae Radix Alba samples and identify common peaks. The hepatoprotective effect was evaluated using the survival rate of HepG2 cells as the pharmacodynamic index. Grey Relational Analysis（GRA），Principal Component Analysis（PCA），and Orthogonal Partial Least Squares-Discriminant Analysis（OPLS- DA）were comprehensively applied to screen potential active components. For the screened active components，multi-dimensional pharmacodynamic verification was further conducted through cell viability assays，morphological observation，and detection of alanine transaminase（ALT）and aspartate transaminase（AST）levels in the supernatant. [Results] The UPLC- Q-TOF-MS fingerprint was successfully established，with 17 common peaks calibrated and the corresponding components identified. Comprehensive analysis by GRA，PCA，and OPLS-DA revealed that gallic acid，oxypaeoniflorin，albiflorin，paeoniflorin，and benzoylpaeoniflorin were the key active components in Paeoniae Radix Alba for attenuating the hepatotoxicity of Tripterygium wilfordii . Cell experiments confirmed that all five compounds could significantly increase the survival rate of HepG2 cells induced by the main hepatotoxic component of Tripterygium wilfordii triptolide（TP），improve cell morphology，and reduce the levels of ALT and AST in the cell supernatant. [Conclusion] By integrating spectrum- effect relationship analysis with multi- dimensional pharmacodynamic verification，this study identified five active components from Paeoniae Radix Alba that attenuate the hepatotoxicity of Tripterygium wilfordii ，providing a scientific basis for elucidating the material foundation of the detoxification effect of Paeoniae Radix Alba in combination therapy and its clinical application. |
| Key words: Paeoniae Radix Alba Tripterygium wilfordii spectrum-effect relationship hepatotoxicity active component UPLC-Q-TOF-MS |
